dryudowskiDr. Guillermo Yudowski

Assistant Professor
Institute of Neurobiology, Lab 309-312
787-721-4149

email: guillermo.yudowski@upr.edu

web: http://theylab.org/

Our main goal is to understand the molecular events leading to and involved in neuropsychiatric disorders. We utilize molecular biology and live-cell imaging techniques to explore the function of single cells in real time. Our main areas of research are:

GPCR (NIH-NIDA)

Our work is focused on the function of G protein-coupled receptors (GPCR) the largest family of signaling receptors regulating neural function and a major target for therapeutic intervention and drug discovery. We investigate the trafficking and signaling in real time of the Mu opioid receptor and the Cannabinoid receptor (CNR1-CNR2).

BK Channels (NIH-NIAAA)

In close colaboration with the Treistman lab. we investigate the physiology and trafficking of the BK channel and its subunits during acute and chronic regulation by ethanol exposure in neurons.

Present Funding: NIH R01

Selected Publications:

  • Jagla CAD, Scott CE, Tang Y, Qiao C, Mateo-Semidey GE, Yudowski GA, Lu D, Kendall DA (2018) Pyrimidinyl Biphenylureas Act as Allosteric Modulators to activate Cannabinoid Receptor 1 and initiate β-Arrestin-dependent Responses. Mol Pharmacol. 2018 Oct 15. pii: mol.118.112854. doi: 10.1124/mol.118.112854. PMID: 30322873
  • Roman-Vendrell C, Yudowski GA (2015) Real-time imaging of mu opioid receptors by total internal reflection fluorescence microscopy. Methods Mol Biol. 1230:79-86 PMID:25293317
  • Roman-Vendrell C, Chevalier M, Acevedo-Canabal AM, Delgado-Peraza F, Flores-Otero J, Yudowski GA (2014) Imaging of kiss-and-run exocytosis of surface receptors in neuronal cultures. Front Cell Neurosci. 8:363 PMID:25404895.
  • Flores-Otero J, Ahn KH, Delgado-Peraza F, Mackie K, Kendall DA, Yudowski GA (2014) Ligand specific endocytic dwell times control functional selectivity of the cannabinoid receptor 1. Nature Communications 5:4589. 45
  • Montiel-Gonzalez MF, Vallecillo-Viejo I, Yudowski GA, Rosenthal JJ (2013) Correction of mutations within the cystic fibrosis transmembrane conductance regulator by site-directed RNA editing. Proc Natl Acad Sci USA 110(45):18285-18290 PMID:24108353.
  • Yudowski GA, Olsen O, Adesnik H, Marek KW, Bredt DS (2013) Acute inactivation of PSD-95 destabilizes AMPA receptors at hippocampal synapses. PLoS One 8(1):e53965 PMID:23342049
  • Roman-Vendrell C, Yu YJ, Yudowski GA. (2012) Fast modulation of mu-opioid receptor (MOR) recycling is mediated by receptor agonists. J Biol Chem 287(18):14782-14791.
  • Yudowski GA. von Zastrow M. (2011) Investigating GPCR endocytosis and trafficking by TIR-FM. Methods Mol Biol 756:325-332.
  • Yudowski GA, Puthenveedu MA, Henry A, von Zastrow M. Cargo-mediated regulation of the Rab4-dependent recycling pathway. Molecular Biology of the Cell.  2009 June 20(11):2774-84
  • Yudowski GA, Puthenveedu MA, von Zastrow M. Real time imaging of discrete exocytic events mediating surface delivery of AMPA receptors. J Neurosci. 2007 Oct 10;27(41):11112-21.
  • Puthenveedu MA, Yudowski GA and Mark von Zastrow. Endocytosis of neurotransmitter receptors: location matters. Cell. 2007 Sep 21;130(6):988-9.
  • Yudowski GA, Puthenveedu MA, von Zastrow M. Distinct modes of regulated receptor insertion to the somatodendritic plasma membrane.  Nat Neurosci. 2006 May;9(5):622-7
  • Yudowski GA, Bar Shimon M, Tal D, Gonzalez-Lebrero R, Rossi R, Garrahan PJ, Beauge LA, Karlish SJ. Evidence for tryptophan residues in the cation transport path at the extracellular surface of the Na+,K+-ATPase. Biochemistry. 2003 Sep 2;42(34):10212-22
  • Yudowski GA, Shimon MB, Gonzalez-Lebrero RM, Rossi RC, Garrahan PJ, Karlish SJ, Beauge L. Extracellularly applied Br-TITU inhibits the Na+/K+ pump by interacting with tryptophan at the entrance to the cation sites. Ann N Y Acad Sci. 2003 Apr;986:287-9
  • Efendiev R, Yudowski GA, Zwiller J, Leibiger B, Katz AI, Berggren PO, Pedemonte CH, Leibiger IB, Bertorello AM. Relevance of dopamine signals anchoring dynamin-2 to the plasma membrane during Na+,K+-ATPase endocytosis. J Biol Chem. 2002 Nov 15;277(46):44108-14
  • Yudowski GA, Efendiev R, Pedemonte CH, Katz AI, Berggren PO, Bertorello AM. Phosphoinositides-3 kinase binds to a proline-rich motif in the Na+, K+-ATPase a-subunit and regulates its trafficking. Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6556-61
  • Ogimoto G, Yudowski GA, Barker CJ, Kohler M, Katz AI, Feraille E, Pedemonte CH, Berggren PO, Bertorello AM. G protein-coupled receptors regulate Na+, K+-ATPase activity and endocytosis by modulating the recruitment of AP-2 and clathrin.  Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3242-7

  • Bertorello AM, Ridge K, Ogimoto G, Yudowski GA, Katz AI, Sznajder JI.  Vectorial movement of sodium in lung alveolar epithelium: Role and regulation on the Na+,K+-ATPase..Acute respiratory distress syndrome: Cellular and molecular mechanisms and clinical management. Plenum Press, New York, NATO ASI series. 1998.