Dr. Loyda M. Meléndez, MT, MS, Ph.D.

Professor
Dr. Loyda M. Meléndez

Contact Info

  • loyda.melendez@upr.edu
  • Department of Microbiology and Zoology,
    University of Puerto Rico-School of Medicine,
    P.O. Box 365067
    San Juan, P.R. 00936-5067
  • 787) 758-2525 xt 2816 xt Lab 2817

Education

  • S. Medical Technology, University of Puerto Rico, Rio Piedras and Medical Sciences Campus , P.R.1979
  • S. Microbiology, University of Georgia, 1981
  • D. Exp. Path/Immunology, Emory University and CDC, Altanta, GA, 1990
  • Post-doctoral training in Immunohematology/Cytometry, Emory University Pathology Dept, Altanta, GA, 1991 ; and in Pediatrics Infectious Diseases: Role of the placenta in HIV transmission, Emory University Pediatrics, Altanta, GA, 1992

ACADEMIC PROFILES

ORCID: https://orcid.org/my-orcid?orcid=0000-0003-2571-8206
LINKEDIN: https://www.linkedin.com/in/loyda-melendez-81b8a315/
Bibliography: http://www.ncbi.nlm.nih.gov/sites/myncbi/loyda.melendez.1/bibliography/41159254/public/?sort=date&direction=ascending
PubMED http://www.ncbi.nlm.nih.gov/pubmed/?term=melendez-guerrero+LM
Researchgate: https://www.researchgate.net/profile/Loyda-Melendez
Scholar: https://scholar.google.com/citations?view_op=new_articles&hl=en&imq=Loyda+Melendez#
Translational Proteomics Center : https://rcmi.rcm.upr.edu/proteomic-page

MISSION AND FIELD OF INTEREST

Laboratory Mission

To prepare undergraduate and graduate students in the field of Viral Innate Immunity focusing on the role of macrophages in HIV infection and pathogenesis. To apply proteomics to uncover the molecules and signaling pathways activated by viruses (HIV, Zika, SARSCoV2) to find targets for diagnosis and therapy against viral diseases.

Field of Interest: 

Viral Innate Immunity, Neurosciences, and Proteomics

Active collaborations and projects

Ongoing Research Support

  • U54MD007600-29 (Fernandez-Repollet, PI) 1.2 cal mo  8/01/22-7/31/2025
    RCMI Translational Proteomics Center- Research Capacity Core
    The major goals of this activity are to expand our research core capabilities in Clinical and Basic Science Proteomics that would lead to improved diagnosis and therapeutic monitoring in health disparities.
    Role: Activity Director
  • NCRR-P20 RR016470 Rodriguez-Medina (PD) 1.2 cal mo 8/01/21 – 5/30/2026
    Advancement of Biomedical Research Excellence (INBRE)
    Clinical Proteomics Discovery Core Facility at University of Puerto Rico Medical Sciences Campus provides new resources for the Advancement of Biomedical Research Excellence. This activity provides funds for developing workshops for faculty and students in the island of Puerto Rico.
    Role: Core Leader

Completed Research Support (Last 5 years)

  • U54MD007600-29 (Fernandez-Repollet 1.2 cal mo 8/01/17-7/31/2022
    RCMI Translational Proteomics Center
    The major goals of this activity are to expand our research core capabilities in Clinical and Basic Science Proteomics that would lead to improved diagnosis and therapeutic monitoring in health disparities.
    Role: Activity Directo
  • UPR  0.5 cal mo  Melendez (PI) 8/01/2020-12/31/2021
    Host factors associated with COVID-19 disease severity.
    The major goals of this project are to define the genetics determinants and innate defenses impact COVID-19 disease severity.
  • SC1GM113691-04 (NIGMS) Melendez (PI)   6.0 cal mo  5/01/15-4/30/2021
    Targeting Monocyte/macrophage cathepsin B interactome in HIV-1 neurocognitive disorders.
    This project explored the role of neurotoxic proteins from macrophages in the development of HIV-neurotoxicity and their expression in in patients treated with combined antiretroviral therapy or cART. HIV.

Students

PAST AND CURRENT STUDENTS MENTORED IN RESEARCH FACULTY MEMBER

Name of Past and Current Students Undergraduate or Graduate Student1 Period of Time Mentored by Faculty Member2 Prior Academic Experience or Degree Earned3

Degree  Yr Institution

 Student’s Current Support Position or Source of Support

Past Students

        
1.    Marinés Plaud (G) 1993-1996 MS-1996 UPR-MSC MS Research Associate

UPR-MSC
2.    Jessicca Renta (G) 1994-1997 MS-1997 UPR-MSC MS Research Associate-UPR MSC
3.    Johana Meléndez (G) 1996-1998 MS-1998 UPR-MSC MS Flow Cytometry  Assistant Director-Moffit Cancer Center
4.    Fernando Soto (UG) 1994-1995 BS-1995 UPR-Rio Piedras MD MSC

 
5.    Monica Grafals (UG) 1995 BS-1998 UPR-Rio Piedras MD MSC

 
6.    Gill Nieves (UG) 1996-1997 BS-1998 UPR-Rio Piedras MD MSC
7.    Zulma Carrasquillo (UG) 1997 UPR-Rio Piedras PhD
8.    Robert Bowles (UG) 1999 UPR-Rio Piedras MD
9.    Mariela Agosto (UG) 2000 UPR- Cayey MBRS
10.  Yaritza Puig (UG) 2000 UPR-Rio Piedras MBRS
11.  Gisela Torres Harvard Medical Student 1999-2000 UPR-Mayaguez M.D. U. Texas
12.  Zulma Sánchez (UG) 2000-2001 U.de Colombia U.de Colombia
13.  Rafael Contreras (UG) 2000-2001 BS. 2001

U. of Colombia, SA

 Post-Doctoral

US
14.  Sylvia Berríos (UG) Summer-2001 UPR-Humacao MD
15.  Johanna Méndez (UG) Summer-2001 UPR-Bayamón MD
16.  Miguel Arroyo (G) 1996-2001 Ph.D. 2001 PhD Captain

Walter Reed Army Research Institute
17.  Manuel Santiago (UG) 2000-2001 BS MS-School of Public Health
18.  Johanna Díaz (G)

 

 Summer-2001 UPR-MSC PhD
19.  Aksim G. Rivera (UG) 2001 UPR-Rio Piedras UG-UPR System
20.  Lynnette López (UG) 2001 UPR-Bayamón MD PhD student
21.  Lourdes Rodriguez (UG) Summer 2004 UPR-Humacao UG-UPR System
22.  Edwin Sánchez (G) 1998-2003 Ph.D. 2003

UPR-MSC

 PhD Research Scientist

Abbott Labs
23.  Pedro Vélez (G) 2001-2003 UPR-MSC Master, MD
24.  Dianedis Toro (G) 2001-2008 Ph.D.UPR-MSC(RISE) PhD Faculty UPR Bayamón
25.  Claribel Luciano (G) 2003-2008 Ph.D. UPR-MSC(RISE) PhD Faculty SJB
26.  Ixane Velazquez (G) 2005-2008 MS UPR-MSC MS AMGEN
27.  Juliana Perez Laspiur, PhD Postdoc 2006-2015 U of Michigan Faculty UPR Aguadilla
28.  Lynette Bosques (UG) Summer 2006 UPR-MSC MD-PhD-US- Yale U
29.  Stephanie Dorta (UG) 2006-2007 UPR-Rio Piedras PhD Assistant Professor UPR-MSC

Dept of Microbiology
30.  Katia Garcia (G) 2003-2009 PhD 2009 UPR-MSC(RISE) PhD USDA
31.  Rafael Romeu (UG) 2009-2010 UPR-Rio Piedras MD
32.  Christian Avilés (UG) 2010-present UPR-Rio Piedras MD
33.  Maria del Carmen Lopez (UG) 2010-present UPR-Rio Piedras MD
34.  Eillen Rodriguez (G) 2007-2011 Ph.D. UPR-MSC(RISE) PhD Scientific Manager AMGEN
35.  Silvia Kares Lawyer 2010-2011 UPR-Rio Piedras MD
36.  Viviana Melendez (UG) Summer 2011 UPR-Mayaguez Medical School- UCC
37.  Raymond Quiles (UG) 2011-present Interamericana Graduate School US
38.  Gisela Delgado (UG) 2011-present UPR-Rio Piedras MD San Juan Bautista
39.  Frances Zenón (G) 2010-2014 Ph.D. UPR-MSC(RISE) Ph.D. Faculty
40.  Mariangeline Gonzalez (UG) 2013-2014 UPR_Rio Piedras MD- Researcher at UPR-MSC
41.  Linda Rivera, PhD Postdoc 2009-2014 RCM Faculty, UAGM-Carolina
42.  Israel Mendez (UG) 2013-2014 UPR_Rio Piedras MD UPR-MSC
43.  Natalia Hernandez (UG) 2013-2014 UPR-Rio Piedras MD UPR- MSC
44.  Frances Acevedo (UG) 2013-2015 UPR-Rio Piedras Ph.D. U. of Michigan Graduate School
45.  Karla Pagán (UG) 2014-2016 UPR-Bayamón MD
46.  Alexandra Ogando (UG) 2014-2016 UPR-Rio Piedras MD
47.  Krystal Colon (G) 2009-2015 Ph.D. UPR-MSC (RISE) Ph.D. Pfizer
48.  Yisel Cantres (G)

Post doc

 2010-2015

2015-2017

 Ph.D. UPR-MSC (RISE) Postdoc, UPR-MSC, Assistant Professor Dept of Microbiology UPR-MSC
49.  Kristal M. Delgado Serrano (UG) Summer 2015 UPR- Mayagüez Undergraduate Student
50.  Nicole Marzán Rivera (UG) Ph.D. UPR-MSC Researcher at a Provete Industry
51.  Eraysy Machin (UG) 2016-2017 UPR-Rio Piedras MD UPR-MSC
52.  José Sanchez (UG) 2017-2018 UPR-Rio Piedras Biol 4990 student
53.  Manuel Alvarez (UG) 2017-2018 UPR-Rio Piedras PhD Bioinformatics
54.  Aemil Santos (UG) 2017-2018 UPR-Bayamon Amgen
55.  Camille Zenón (G) 2014-2019 MS UPR-MSC Research Technician, MSC
56.  Omar Vélez (G) 2014-2019 PhD UPR-MSC(RISE) PhD Assistant Professor UPR-Ponce
57.  Estheysy Roman Pagan (UG) 2018-2019 UNE Research  student
58.  Adriadna Ramirez (UG) 2019-2020 UPR-Rio Piedras Medical School Student
59.  Jean Perez Vargas (UG) 2019-2020 UPR-Rio Piedras Medical School Student
60.  Andrea Gonzalez (UG) 2019-2020 UPR-Rio Piedras Biol 4990
61.  Julio Rosado-Philipi (UG) 2019-2021 UPR-Rio Piedras Medical School Student and Volunteer
62.  Lester Rosario (G) 2015-2021 PhD UPR-MSC (RISE) NIH F99/K00, ex-RISE, Doctoral student
63.  Luis Garcia Requeña (UG) 2019-2021 UPR-Rio Piedras Medical School Student
64.  Luz Cartagena (UG) 2017-2021 STEP-UP

UPR-Rio Piedras

 Medical School Student, Guadalajara
65.  Juan C Fipo Cuadrado (UG) 2018-2020 UPR-Bayamon Medical School Student
66.  Gabriel Borges (G) 2015-2023 PhD UPR-MSC (RISE) Postdoc CCC
67.  Veronica Rivera Nieves (UG) 2020-2023 UPR-Rio Piedras Maestria en Salud Publica
68.  Alexandra Rosa (UG) 2022-2024 UPR-Rio Piedras Medical Student Ponce Health Sciences
69.  Alana Mera (UG) Summer 2024 UPR-Rio Piedras STEP-UP
Present students
Luisa Rodriguez (UG) 2022-present UPR-Rio Piedras Biol 4990
Jorlyann (UG) Summer 2025 UPR-Cayey PR-INBRE STCE
Xandra Pena Diaz (UG) Summer 2025 UPR-Cayey CBIOL 5990
Franco Roark (UG) Summer 2025 Mason University CBIOL 5990
Nicolas Miranda Diaz (G) Summer 2025 UPR-Rio Piedras CBIOL 5990

PUBLICATIONS

  1. Melendez-Guerrero LM, Nicholson JK, and MacDougal JS. 1990. In vitro infection of monocytes with HIVBa-L. Effect on cell surface expression of CD4, CD14, HLA-DR, and HLA-DQ. AIDS Res Hum Retroviruses. 1990 Jun;6(6):731-41.PMID:1694679
  2. Melendez-Guerrero L, Nicholson J, and MacDougal S. 1991. In vitro infection of Human Monocytes with HIV-1Ba-L. Effect on Accessory Cell Function for T-Cell Proliferation In Vitro. AIDS Res and Human Retroviruses 7(5): 465-474. PMID: 1873081.
  3. Renta JY, Cadilla CL, Vega ME, Hillyer GV, Estrada C, Jiménez E, Abreu E, Méndez I, Gandía J, Meléndez-Guerrero LM. 1997. Longitudinal studies on maternal HIV-1 variants by biological phenotyping, sequence analysis and viral load. Cell Mol Biol 43(7):1097-114. PMID: 9449544
  4. Plaud-Valentin M, Delgado R, Garcia V, Zorrilla C, Gandia J, Meléndez-Guerrero LM. HIV infection of placental macrophages: effect on the secretion of HIV stimulatory cytokines. Cell Mol Biol (Noisy-le-grand). 1999 Jun;45(4):423-31. PMID: 10432189.
  5. Torres G, García V, Sánchez E, Segarra A, Patterson BK, Meléndez-Guerrero LM.Expression of the HIV-1 co-receptors CCR5 and CXCR4 on placental macrophages and the effect of IL-10 on their expression. Placenta. Suppl A:S29-33. PMID: 11312625.
  6. Sanchez-Carrasquillo E, García V, Rivera CE, Febo I, Melendez-Guerrero LM. 2000. CCR5 and beta-chemokines in HIV-1 infected children. P R Health Sci J. 19(4):345-51. PMID: 11300123
  7. Melendez-Guerrero LM, Arroyo MA, Vega ME, Jimenez E, Hillyer GV, Cadilla CL.2001. Characterization of HIV isolates from Puerto Rican maternal-infant pairs reveal predominance of non-syncytium inducing (NSI) variants with CCR5 genotype. Cell Mol Biol (Noisy-le-grand). 47; 39-47. PMID: 11936872.
  8. Meléndez J, García V, Sánchez E, Delgado R, Torres G, Meléndez-Guerrero LM. 2001. Is decreased HIV-1 infectivity of placental macrophages caused by high levels of beta-chemokines? Cell Mol Biol (Noisy-le-grand). 47 OL51-9. PMID:11936874
  9. Ruff MR, Melendez-Guerrero LM, Yang QE, Ho WZ, Mikovits JW, Pert CB, Ruscetti FA. 2001. Peptide T inhibits HIV-1 infection mediated by the chemokine receptor-5 (CCR5). Antiviral Res. 2001 Oct;52(1):63-75. PMID:11530189

 

  1. Arroyo MA, Tien H, Pagán M, Swanstrom R, Hillyer GV, Cadilla CL, Meléndez-Guerrero LM. 2002. Virologic risk factors for vertical transmission of HIV type 1 in Puerto Rico. AIDS Res Hum Retroviruses. 18(6):447-60. PMID: 11958688.
  2. Luo X, Carlson KA, Wojna V, Mayo R, Biskup TM, Stoner J, Anderson J, Gendelman HE, Meléndez LM. Macrophage proteomic fingerprinting predicts HIV-1-associated cognitive impairment. Neurology. 2003 Jun 24;60(12):1931-7. PMID:12821735.
  3. Wojna V, Carlson KA, Luo X, Mayo R, Meléndez LM, Kraiselburd E, Gendelman HE. 2004. Proteomic fingerprinting of human immunodeficiency virus type 1-associated dementia from patient monocyte-derived macrophages: A case study. J Neurovirol. 2004;10 Suppl 1:74-81. PMID:14982743.
  4. Kamara P, Melendez-Guerrero L, Arroyo M, Weiss H, Jolly P. 2005. Maternal plasma viral load and neutralizing/enhancing antibodies in vertical transmission of HIV: a non-randomized prospective study. Virol J. 2:15. PMID:15730556.
  5. Wojna V, Skolasky RL, Hechavarría R, Mayo R, Selnes O, McArthur JC, Meléndez LM, Maldonado E, Zorrilla CD, García H, Kraiselburd E, Nath A. 2006. Prevalence of human immunodeficiency virus-associated cognitive impairment in a group of Hispanic women at risk for neurological impairment. J Neurovirol. 2006 Oct;12(5):356-64. PMID:17065128
  6. Wojna V, Skolasky RL, McArthur JC, Maldonado E, Hechavarria R, Mayo R, Selnes O, Ginebra T, de la Torre T, Garcia H, Kraiselburd E, Melendez-Guerrero LM, Zorrilla CD, Nath A. 2007. Spanish validation of the HIV dementia scale in women. AIDS Patient Care STDS. 2007 Dec;21(12):930-41. PMID:18154490.
  7. Nieves DM, Plaud M, Wojna V, Skolasky R, Meléndez LM. Characterization of peripheral blood human immunodeficiency virus isolates from Hispanic women with cognitive impairment. J Neurovirol. 2007 Aug;13(4):315-27. PMID: 17849315.
  8. Rozek W, Ricardo-Dukelow M, Holloway S, Gendelman HE, Wojna V, Melendez LM, Ciborowski P. 2007. Cerebrospinal fluid proteomic profiling of HIV-1-infected patients with cognitive impairment. J Proteome Res. 2007 Nov;6(11):4189-99. Epub 2007 Oct 12. PMID: 17929958.
  9. Wojna V, Robles L, Skolasky RL, Mayo R, Selnes O, de la Torre T, Maldonado E, Nath A, Meléndez LM, Lasalde-Dominicci J. 2007. Associations of cigarette smoking with viral immune and cognitive function in human immunodeficiency virus-seropositive women. J Neurovirol. 2007 Dec;13(6):561-8. PMID:18097887.
  10. Laspiur JP, Anderson ER, Ciborowski P, Wojna V, Rozek W, Duan F, Mayo R, Rodríguez E, Plaud-Valentín M, Rodríguez-Orengo J, Gendelman HE, Meléndez LM. CSF proteomic fingerprints for HIV-associated cognitive impairment. J Neuroimmunol. 2007 Dec;192(1-2):157-70. Epub 2007 Oct 22. Erratum in: J Neuroimmunol. 2008 Dec 15;205(1-2):161. PMID:17950469.
  11. Luciano-Montalvo C, Ciborowski P, Duan F, Gendelman HE, Meléndez LM. Proteomic analyses associate cystatin B with restricted HIV-1 replication in placental macrophages. Placenta. 2008 Dec;29(12):1016-23. PMID: 18951626
  12. Luciano-Montalvo C, Meléndez LM. Cystatin B associates with signal transducer and activator of transcription 1 in monocyte-derived and placental macrophages. Placenta. 2009 May;30(5):464-7. PMID: 19342095.
  13. García K, García V, Pérez Laspiur J, Duan F, Meléndez LM. Characterization of the placental macrophage secretome: implications for antiviral activity. Placenta. 2009 Feb;30(2):149-55. PMID: 19070362
  14. García-Crespo K, Cadilla C, Skolasky R, Meléndez LM. Restricted HIV-1 replication in placental macrophages is caused by inefficient viral transcription. J Leukoc Biol. 2010 Apr;87(4):633-6. PMID: 20042472.
  15. Toro-Nieves D (mentee, LM), Perez Laspiur J, Ciborowski P, Duan F, Wojna V, Meléndez LM. 2009. Proteomic analysis of HIV-1 infected monocyte-derived macrophage following infection with primary viral strains isolated from Hispanic women with cognitive impairment”. J NeuroVirology 1:36-50.
  16. Velázquez I (Mentee, LM), Plaud M, Pérez-Laspiur J, Skolasky R, Wojna V and Meléndez LM. 2009. Antioxidant enzyme dysfunction in monocytes and CSF of Hispanic women with HIV-associated cognitive impairment. J Neuroimmunol 209:206-211
  17. García, K (Mentee, LM), García, V, Duan, F, Perez Laspiur J, Meléndez, L.M. 2009. Characterization of the placental macrophage secretome: Implications for antiviral activity. 30: 149-155. PMID: 19070362
  18. Kraft-Terry S, Gerena Y, Wojna V, Plaud-Valentin M, Rodriguez Y, Ciborowski P, Mayo R, Skolasky R, Gendelman HE, Melendez LM. Proteomics analysis of monocytes from Hispanic women with HIV-associated dementia show depressed antioxidants. J. Proteomics and Clinical Applications 4:710-714. PMID:21137088
  19. Peters J, Dieppa-Perea L, Melendez LM*, Quirk GJ. 2010. Induction of fear extinction with hippocampus infralimbic BDNF. Science 238:1288-1290. PMID: 20522777.
  20. Melendez LM*, Colon K, Rivera L Rodriguez-Franco E, and Toro-Nieves D. (Invited Review) 2011. Proteomic analysis of HIV-infected macrophages. J Neuroimmune Pharmacol. 6:89-106. PMID: 21153888.
  21. Rivera-Rivera L, Perez-Laspiur J, Colon, K, and Melendez, LM*. Inhibition of interferon response by cystatin B: Implication in HIV replication of macrophage reservoirs. J Neurovirology 18:20-9. PMID: 22147503.
  22. Rodriguez E, Cantres Y, Plaud M, Romeu R, Rodriguez Y, Skolasky R, Melendez V, Cadilla C, and Melendez LM*. 2012. Dysregulation of macrophage-secreted cathepsin B contributes to HIV-1-linked neuronal apoptosi PLoS ONE 7(5): PMID: 22693552.
  23. Cantres-Rosario Y, Plaud-Valentin M, Gerena Y, Skolasky R, Wojna V, Meléndez LM*. Dysregulation of cathepsin B and its inhibitor cystatin B in monocytes and CSF from women with HIV-associated neurocognitive impairment. AIDS 27:347-356. PMID: 23291538.
  24. Rivera LE, Colon K, Cantres YM, Zenon FM, and Melendez, LM. Macrophage derived cystatin B/cathepsin B in HIV replication and neuropathogenesis. Current HIV Research 12: 111-120. PMID:24862331.
  25. Agsalda-Garcia M, Shiramizu B, Melendez LM*, Plaud M, Liang C-Y, Wojna V. Different levels of HIV DNA copy numbers in cerebrospinal fluid cellular subsets. Journal of Health Care for the Poor and Underserved, 24(4) Suppl 8-16 doi: 10.1353/hpu.2014.0010. PMID: 24241256; PMCID: PMC3844931.
  26. Zenon F, Segarra A, Gonzalez M, and Meléndez LM*, Cocaine Potentiates Cathepsin B Secretion and Neuronal Apoptosis from in HIV-infected Macrophages. J Neuroimmune Pharmacology. Published on line ahead of time DOI 10.1007/s11481-014-9563-z
  27. Vázquez-Santiago F, García Y, Rivera-Román I, Noel RJ Jr, Wojna V, Meléndez LM, Rivera-Amill V. Longitudinal Analysis of Cerebrospinal Fluid and Plasma HIV-1 Envelope Sequences Isolated From a Single Donor with HIV Asymptomatic Neurocognitive Impairment. J Virol Antivir Res. 2015;4(1). doi: 10.4172/2324-8955.1000135. PMID: 26167513. July, 2015
  28. Zenón F, Cantres-Rosario Y, Adiga R, Gonzalez M, Rodriguez-Franco E, Langford D, Melendez LM. HIV-infected microglia mediate cathepsin B-induced neurotoxicity. J Neurovirol. 2015 Jun 20. [Epub ahead of print] PMID: 26092112.
  29. Zenón F, Jorge I, Cruz A, Suarez E, Segarra AC, Vázquez J, Meléndez LM, Serrano H. O18 proteomics reveal increased Human Apolipoprotein CIII in Hispanic HIV-1 positive women with HAART that use cocaine. Proteomics Clin Appl. 2015 Aug 10. doi: 10.1002/prca.201400204. [Epub ahead of print] PMID: 26255783.
  30. Colon K, Perez-Laspiur J, Quiles R, Rodriguez Y, Wojna V, Shaffer SA, Leszyk J, Skolasky RL Jr, Melendez LM. Macrophage secretome from women with HIV-associated neurocognitive disorders. Proteomics Clin Appl. 2015 Jul 29. doi: 10.1002/prca.201400203. [Epub ahead of print PMID: 26220577.
  31. Colon K, Vázquez-Santiago F Rivera-Amill V, Delgado G, Massey SE, Wojna V, Noel RJ Jr6 and Meléndez LM. 2015. HIV Gp120 Sequence Variability Associated with HAND in Hispanic Women. J Virology and Antiviral Research. doi: 10.4172/2324-8955.1000135. Epub 2015 Oct 6.
  32. Cantres-Rosario YM, Hernandez N, Negron K, Perez-Laspiur J, Leszyk J, Shaffer SA, Melendez LM. Interacting partners of macrophage-secreted cathepsin B contribute to HIV-induced neuronal apoptosis. 2015 Jul 23. [Epub ahead of print] PMID: 26208400.
  33. Delgado-Velez M, Baez-Pagan C, Gerena Y, Quesada O, Santiago-Perez L, Capo-Velez C, Wojna V, Melendez L, Leon-Rivera R, Silva W, and Lasalde-Dominicci JA. The a-7nicotinic receptor is upregulated in immune cells from HIVseropositive women: Consequences to the cholinergic anti-inflammatory response. Clin and Translational Immunology 4, e53. Dec, 2015
  34. Rivera LE, Kraiselburd E, Meléndez LM. Cystatin B and HIV regulate the STAT-1 signaling circuit in HIV-infected and INF-β-treated human macrophages. J Neurovirol. 2016 Oct;22(5):666-673. PMID:
  35. 27137788. Epub 2016 May 2.
  36. Cantres-Rosario YM, Acevedo-Mariani FM, Pérez-Laspiur J, Haskins WE, Plaud M, Cantres-Rosario YM, Skolasky R, Méndez-Bermúdez I, Wojna V, Meléndez LM. Microwave & magnetic proteomics of macrophages from patients with HIV-associated cognitive impairment. PLoS One. 2017 Jul 26;12(7):e0181779. doi: 10.1371/journal.pone.0181779. eCollection 2017.
  37. Martínez-Rivera FJ, Pérez-Laspiur J, Santiago-Gascot ME, Alemán-Reyes AG, García-Santiago E, Rodríguez-Pérez Y, Calo-Guadalupe C, Otero-Pagán I, Ayala-Pagán RN, Martínez M, Cantres-Rosario YM, Meléndez LM, Barreto-Estrada JL. 2017. Differential protein expression profile in the hypothalamic GT1-7 cell line after exposure to anabolic androgenic steroids. PLoS ONE Jul 18;12(7): e0180409. PMID: 28719635.
  38. Rosario-Rodriguez LJ, Colón K, Borges-Vélez G, Negrón K,Meléndez LM. Dimethyl fumarate prevents HIV-induced lysosomal dysfunction and cathepsin B release from macrophages. J Neuroimmune Pharmacol. 2018 Jul 9. doi: 10.1007/s11481-018-9794-5. [Epub ahead of print] PMID:29987592.
  39. Rosas-Vidal L, Lozada V, Cantres-Rosario Y, Vega-Medina A, Melendez L, and Quirk G. 2018. Alteration of BDNF in the medial prefrontal cortex and the ventral hippocampus impairs extinction of avoidance. Neuropsychopharmacology. Manuscript #NPP-17-1265RR
  40. López, O. V., Gorantla, S., Segarra, A. C., Norat, M. C. A., Álvarez, M., Skolasky, R. L., & Meléndez, L. 2019. “Sigma-1 Receptor Antagonist (BD1047) Decreases Cathepsin B Secretion in HIV-Infected Macrophages Exposed to Cocaine”. Journal of Neuroimmune Pharmacology, 1-15.
  41. Cantres-Rosario, YM. Santos A, Ortiz-Rodriguez S., Santos A, Plaud M, Negron K, Cotto B, Langford D, and Melendez LM. HIV-infected macrophages induce extracellular cathepsin B uptake and damage to neurons. Nature Sci Rep. May 29;9(1):8006. doi: 10.1038/s41598-019-44463-1.
  42. Schmoker AM, Perez Pearson LM, Cruz C, Colon Flores LG, Branfeild S, Pagán Torres FD, Fonseca K, Cantres YM, Salgado Ramirez CA,Melendez LM, Ballif BA, Washington AV. 2020. Defining the TLT-1 interactome from resting and activated human platelets. J Proteomics. 2020 Mar 20;215:103638. doi: 10.1016/j.jprot.2020.103638. Epub 2020 Jan 8.PMID: 31923473
  43. Borges-Velez G, Rosario-Rodriguez L, Rosado-Philippi JE, Cartagena LJ, Garcia-Requena L, Gonzalez A, Perez J, and Melendez 2020. SARS-CoV-2:Biology,Detection, Macrophage Mediated Pathogenesis and Potential Treatments. Virol Immunol J. 2020;4(2):242. doi: 10.23880/vij-16000242. Epub 2020 Jul 2.PMID: 34485853
  44. Martínez–Matías N, Chorna N, González–Crespo S, Villanueva L, Montes–Rodríguea I, Melendez-Aponte LM, Roche–Lima A, Carrasquillo–Carrión K, Santiago-Cartagena E, Rymond BC, Babu M, Stagljar I, and Rodríguez–Medina JR. 2021. Toward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiaevia integrative multi-OMICs analysis. Scientific Reports Apr 1;11(1):7411. DOI: 10.1038/s41598-021-86671-8. PMID: 33795741 PMCID: PMC8016984
  45. Borges-Vélez G, Rosado-Philippi J, Cantres-Rosario YM, Carrasquillo-Carrion K, Roche-Lima A, Pérez-Vargas J, González-Martínez A, Correa-Rivas MS, and Meléndez LM. Zika virus infection of the placenta alters extracellular matrix proteome. J Mol Histol. doi: 10.1007/s10735-021-09994-w. Online ahead of print.PMID: 34264436
  46. Zenon-Melendez CN, Carrasquillo Carrión K, Cantres Rosario Y, Roman E, Roche Lima A, & Meléndez LM. Inhibition of cathepsin B and SAPC secreted by HIV-infected macrophages reverse common and unique apoptosis pathways. J of Proteome Research January 7. https://doi.org/10.1021/acs.jproteome.1c00187
  47. Rosario-Rodriguez LJ, Gerena Y, Cuadrado JC, Cartagena LJ, and Meléndez LM. Cannabinoid receptor type 2 agonist JWH-133 decreases cathepsin B secretion and neurotoxicity from HIV-infected macrophages. 2022. Nature Sci. Rep. Jan 7;12 (1):233. PMID: 34996989
  48. Bidlack JM, Chang SL, Fitting S, Gendelman HE, Gorantla S, Kumar S, Garibaldi Marcondes MC, Meigs DD, Meléndez LM, Sariyer IK, Yelamanchili S. 2022. The COVID-19 Pandemic: Reflections of Science, Person, and Challenge in Academic Research Settings. J Neuroimmune Pharmacology https://doi.org/10.1007/s11481-021-10035-2
  49. Borges-Vélez G, Arroyo JA, Cantres-Rosario YM, Rodriguez de Jesus A, Roche-Lima A, Rosario-Rodriguez L, Correa-Rivas MS, Campos-Rivera M, Rosado-Philippi J, and Meléndez LM. 2022. Decreased CSTB, RAGE and Axl receptor is associated with Zika infection in the human placenta. Cells 11, 3627. https://doi.org/10.3390/cells11223627
  50. Bayrón-Marrero Z, Branfield S, Menéndez-Pérez J, Nieves-López B, Ospina C, Cantres-Rosario Y, Melendez LM, Robert Hunter R, Gibson A, Maldonado-Martínez G, and Washington AV. The Characterization of Soluble Triggering Receptor Expressed on Myeloid Cells Like-Transcript-1 (sTLT-1) fragment as a biomarker for Stable Cardiovascular Diseases. 2023. Research and Practice in Thrombosis and Haemostasis. J. Mol. Sci.24(17), 13632; https://doi.org/10.3390/ijms241713632
  51. Rosario-Rodriguez LJ, Cantres-Rosario YM, Carrasquillo-Carrion K, Rodriguez de Jesus A, Garcia-Requeña L, Roche-Lima A, and Meléndez 2024. Quantitative proteomics reveal that CB2R agonist JWH-133 Downregulates NF-kappaB activation, oxidative stress, and lysosomal exocytosis from HIV infected macrophages. Int J Mol Sci. 13;25(6):3246. doi: 10.3390/ijms25063246.PMID: 38542221.
  52. Rosario-Rodríguez LJ, Cantres-Rosario YM, Rodríguez-De Jesús AE, Carrasquillo-Carrión K, Méndez LB, Rivera-Nieves V , Rosa-Diaz A, Roche Lima A, Bertran J, Meléndez, LM. Plasma proteins associated with COVID-19 severity in Puerto Rico. Int J Mol Sci.25(10), 5426; https://doi.org/10.3390/ijms25105426
  53. Vélez-López O, Carrasquillo-Carrión K, Yadira M. Cantres-Rosario, Machín-Martínez E, Álvarez-Rios ME, Roche-Lima A, Tosado-Rodríguez EL, Meléndez LM. Analysis of Sigma‐1 Receptor Antagonist BD1047 Effect on Upregulating Proteins in HIV‐1‐Infected Macrophages Exposed to Cocaine Using Quantitative Proteomics. Biomedicines 12,1934. https://doi.org/ 10.3390/biomedicines12091934
  54. Rivera-Serrano, M; Flores-Colón, M; Valiyeva, F; Meléndez, LM; Vivas-Mejia, P. 2025. Upregulation of MMP3 promotes cisplatin resistance in ovarian cancer. J. Mol. Sci. 26(9), 4012; https://doi.org/10.3390/ijms26094012
  55. Morales-Cabán BM, Cantres-Rosario YM, Tosado-Rodríguez EL, Roche-Lima A, Meléndez LM, Boukli NM, and Suarez-Arroyo IJ. SCAMP3-Driven Regulation of ERK1/2 and Autophagy Phosphoproteomics Signatures in Triple-Negative Breast Cancer. Submitted to Cells. May 25, 2025.
  56. Armina-Rodriguez, Valdez BN, Ocasio-Malavé C, Cantres-Rosario YM, Carrasquillo Carrión K, Meléndez LM, Roche-Lima A, Tosado E, Espino AM. Quantitative Proteomics Reveals Fh15 as an Antagonist of TLR4 Downregulating the Activation of NF-κB, Inducible Nitric Oxide, Phagosome Signaling Pathways, and Oxidative Stress of LPS-stimulated Macrophages. J. Mol. Sci.2025, 26(14), 6914; https://doi.org/10.3390/ijms26146914

BOOK CHAPTERS / TECHNICAL REPORTS

  • Gendelman, H, Anderson E, Melendez, L, and Zheng, J. Chemokines and their receptors in HIV-1 neuropathogenesis. 2006. In Vivo Models of HIV Disease and Control. Eds H. Friedman, S. Specter, and M. Bendinelli. Springer. p. 45-80.
  • Melendez, L.M., Luciano, C. Mayo-Santana R., Wojna, V. Ethnicity and Neuro-AIDS Conditions in the HAART Era. 2009. In “The Spectrum of NeuroAIDS Disorders: Pathophysiology, Diagnosis, and Treatment”. ASM Press. p. 427-441.

SELECTED HONORS/AWARDS

  1. Fellowship from the government of Puerto Rico (Fomento Industrial) for studies leading to M.S in Industrial Microbiology, 1979-81.
  2. Minority Access Research Career faculty fellowship from NIH for doctoral studies, 1985-89.
  3. Co-investigator in an NIH funded R0-1 to study the mechanisms of transplacental transmission of HIV, 1991-92.
  4. Principal investigator in a two-year project entitled: “Role of the placenta in HIV-1 vertical transmission”, 1993-95.  Sponsored by the Deanship of Academic Affair “CIDIC”.
  5. Activity coordinator in a NIH-RCMI funded research grant entitled: “HIV-1 Env Gene variation and tropism in vertical transmission, RR-03051, 1994-98.
  6. Travel Award from NIH to Keystone symposia entitled: “NIH-AIDS Postdoctoral Fellows meeting”.  Albuquerque, New México, 1993.
  7. Travel Award from NIH-RCMI to First National HIV Meeting in Washington, D.C., 1993.
  8. Principal Investigator in a NIH-MBRS funded grant entitled: “Role of the placenta in vertical transmission of HIV”.  SO6 GM08224.
  9. Award from the Dean of Biomedical Sciences and the Program of High School Teachers and Students (K-12 Program, 1995) for advising students in research projects during the summer. Sponsored by NIH Grant #R25 RR 10852.
  10. Professional – Excellence Award from the Dean of Allied Health School. 1996.
  11. Travel Award for FASEB, Summer Research Conference on “Virus Assembly” Vermont, 1998.
  12. Glaxo-Welcome Research Award in the Category of Researcher with Experience.1999.
  13. Principal Investigator in NIH-SCORE Program funded research grant entitled:  “Role of Chemokine Receptors in HIV-1 infection across the placenta”.  2000-2004. SO6GM08224.
  14. Principal Investigator in NIH-SCORE Program funded research grant entitled:  ” Protein profiling of “putative” anti-retroviral factors in placental macrophages”. 2004-2008. S06GM008224.
  15. Principal Investigator in NIH-Specialized Neuroscience Research Program (SNRP) funded research grant entitled:  ” Monocyte Immunity and HIV Dementia”. 2001-2008
  16. Coordinator in the NIH-RCMI G12RR03051 activity entitled: “Clinical Proteomics Discovery Core Facility”-2006-2011.
  17. Elected Academic Senator from the School of Medicine 2008-2010.
  18. Principal Investigator in the NIH-RO1 entitled 1R01MH083516 – 01   Modulation of Cystatins and Cathepsins in HIV-1 Neuropathogenesis. 2009-2012.
  19. Honorary Mention for “Monocyte Immunity and HIV Dementia”. Merck Innovation Awards. 2009.
  20. NIH reviewer of a special emphasis panels. “Mechanisms of Drug Abuse Interactions with HIV Neuropathogenesis” and “Alcohol abuse Conference R13/U13 Grants” (2007-2009).
    1. Martin Delaney R01 proposals on HIV Reservoirs; HIV and Aging (2011-2012).
    2. NIMH P01 Novel NeuroAIDS Therapeutics: Integrated Preclinical/Clinical Program (March 2014).
  21. Key Proteomics Director in the NIMHHD 8G12-MD007600 activity entitled: “Translational Proteomics Center. 2006-2016.
  22. Invitations to present research at RCMI meeting 2012 and Temple University Oct 2012.
  23. HiREC Advisory Committee (2013-present)
  24. Research Advisory Committee to the Chancellor (2010-2014)
  25. Search Committee for a new Dean of the School of Medicine (August- October 2014).
  26. Principal Investigator in NIH-NIGMS SC1 “Targeting Macrophage derived cathepsin 1 in HIV neurocognitive disorders” May 2015-January 2019.
  27. Invitation for an oral presentation. 2nd International Conference on Retroviruses and Novel Drugs. South Africa, June 30, 2016.
  28. Elected Council of the Society for Neuroimmune Pharmacology- April 2017-2022
  29. RISE Program Progress Report Reviewer May 2014-present
  30. INBRE Program Pilot Proposal Reviewer May 2016-present
  31. INBRE Proteomics Core Facility Coordinator 2016-present
  32. Elected Academic Senator from the School of Medicine 2017-2019.
  33. Elected President of Senate Academic Affairs Committee 2017-2018.
  34. 1st HiREC Award on Excellence in Clinical and Translational Research. May 2019
  35. Invited Reviewer for NIH grants on US and Brazil collaboration. July 2019.
  36. Puerto Rico Society of Microbiologist 63rd Annual Meeting; Premio Arturo Carrión, June 4, 2021
  37. Puerto Rico Health Sciences Journal Associate Editor, August 2021
  38. Abstract Reviewer Medical Sciences Campus Scientific Research Forum March 2022
  39. Research Abstract Committee member of the Hispanic Alliance of Clinical and Translational Research Scientific Day March 2022.
  40. Invited Speaker for 14th UPR RISE Area Conference “Two Years of COVID-19: Lessons and Improvements”.  Embassy Suites Hotel, San Juan, PR. April 9, 2022.
  41. Hispanic Alliance Researcher Productivity Award. Hispanic Alliance of Clinical and Translational Research Scientific Day. Hotel Royal Sonesta, San Juan, PR. May 4, 2022.
  42. Invited reviewer for PlosONE, December 2022
  43. Travel award for the IDeA Proteomics Symposia for Core Directors and Staff. Feb 7-10, 2023
  44. Puerto Rico IDeA Network of Biomedical Research Excellence Pilot proposal reviewer, January 2023
  45. Invited by the Society on NeuroImmune Pharmacology (SNIP) to co-chair Symposium 6 on March 18, 2023, in the 27th SNIP Annual Conference to be held in New Delhi, India from 15th to 18th in March 2023.
  46. RCMI National meeting Abstract Reviewer. April 2023
  47. Invited reviewer for manuscript J of Neuroimmune Pharmacology and Therapeutics. April 2023
  48. Invited reviewer for J of Cellular and Molecular Histology. April 2023
  49. Invited Speaker for University of Vermont Department of Biology. May 1, 2023
  50. NIH Reviewer F31 and F32 HIV training grants ZRG1F17A-J(20) on July, 2023
  51. Nominated for the Sigma Xi Society membership. August 29, 2023
  52. Invited reviewer for Nature Communications, December 2023
  53. NIH Invited reviewer for the HIV Comorbidities and Clinical Studies (HCCS) study section July 9-10, 2024.
  54. Awarded NIH-Step-Up mentoring undergraduate student Ms. Alana Mera. June and July 2024
  55. Invited Reviewer to Journal Aging-June 27, 2024
  56. Invited Reviewer for AIDS journal-July 25, 2024
  57. External Faculty Evaluator, Dept. of Tropical Medicine, Medical Microbiology, and Pharmacology, U. of Manoa, Hawaii-July, 2024
  58. Invited Reviewer for Journal of Neuroimmune Pharmacology- September 1, 2024
  59. Invited Reviewer for MDPI-Proteomes- June 2025

Patents Submitted:

  1. A method to reduce HIV replication in macrophages.
  2. Proteins associated with HIV induced dementia in monocytes and CSF.

Patents Awarded:

  1. A method of reducing HIV-Replication on human body tissue comprising down regulating the expression of the protein Cystatin B on said human body tissue Pat No. 8,143,231. Awarded to Drs. Melendez and Claribel Luciano. 2012.